Detección, Clasificación y Conteo de Leucocitos en Frotis de Sangre Periférica. J . Martínez-Castro* S. Reyes-Cadena** E. Felipe-Riverón *. * Centro de. Scribd is the world’s largest social reading and publishing site. FROTIS DE SANFRE PERIFERICA Un frotis de sangre preparado en forma adecuada es esencial para asegurar la evaluación correcta de la morfología.

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Finally we discuss the results and conclusions of the paper. Leukocyte image segmentation using frots visual attention. Table 2 shows the number of leukocytes of each type present in 45 smears previously classified by a specialist vs. The level to which the subdivision is carried out depends on the problem being solved. Many papers aim at the problem of image analysis of white blood cells in bone marrow microscopic images.

In an adult clinically healthy, it is performed in the bone marrow, continues in the peripheral blood and in some cases ends in tissue from different organs of the human body. In Biomedica l Imagingsange Pueden observarse eritrocitos nucleados.

The task of segmenting them is carried out after the contrast enhancement of the adequately prepared smear and the prior transformation to a gray-level image of the commonly used color RGB image. Manzoni D, Sujobert P.

Frotis de sangre

However, the ground truth in medical imaging is an elusive concept because there is not a clear and absolute way to define it. Similarly the median filter, and closing and frogis operators are applied in order to obtain an image with the least possible noise and the cell membrane well defined Fig.

The effectiveness of our method was tested with a blind experiment. Blood cell image segmentation: Reporting and grading of abnormal red blood cell morphology. Comput Biol Med37 1: After segmenting the nuclei to isolate them from the erythrocytes, we proceed to separate them from the nuclear membrane. With this, we obtained an image with the objects of interest more defined nucleiFig.


Hospital General Universitario Dr. Frotis de sangre [Internet]. After smoothing the image it was observed the presence of some artifacts that are not part of the objects of interest, which must be removed with the help of a median filter, and a couple of morphological filters.

Importancia del estudio del frotis de sangre periférica en ancianos

Hematologic complications of alcohol use [Internet]. Nuclei segmentation The first step was to separate the white blood cells from the red blood cells.

The counting and classification of leukocytes is a task that in most cases until today is carried out sangree by a clinical laboratory technician or hematopathologist who requires years of study and experience and usually uses a manual counter of leukocytes to perform this task.

Medscape [revista en Internet]. In blood smears, the number of red cells is many more than white blood cells. Neutrophils, Eosinophils, Basophils, Monocytes and Lymphocytes.

Wolters Kluwer; [citado 6 Mar ].

Using the k -NN classifier in combination with the first Minkowski metric, in addition to techniques of digital image processing, a computational system platform-independent was able to identify, to classify and to count the five normal types of leukocytes.

ABSTRACT The study of smear of peripheral blood consists on specifying and informing the morphological alterations of blood elements; This is a simple, inexpensive, quick exam in reporting its results, but at the same time requires much care and experience, given the time and interest that is devoted to its learning, the quality of the extension and its staining. Goldman L, Schafer AI. For our objective, it is interesting to know what constitutes the blood, the unique fluidic tissue of our body.

An Med Interna Madrid [revista en Internet]. Imag e Visio n Comput.

Detección, Clasificación y Conteo de Leucocitos en Frotis de Sangre Periférica

Textbook of Clinical Chemistry and Molecular Diagnostic. Then, the processes for the reduction of the additive noise and the contrast improvement are carried out. Generalmente existen varias causas de anemia en el anciano. Morphological operators have been developed to detect and classify other objects, i.


Neutrophils, eosinophils santre basophils are known as granulocytes, because frois present a granulated nucleus, whereas monocytes and lymphocytes present a well-defined one-piece nucleus. Segmentation subdivides an image into its constituent regions or objects. Atlas of peripheral blood. The distance measure used was frltis Manhattan distance. Leukocytes are cells that represent the main mechanism of the body’s defense against infectious processes and inflammatory and allergic reactions.

The leukocytes, better known as white blood cells, lack of pigment. Conganat; [citado 23 Feb ]. The hematopoiesis presents three major cell lines: Erythrocytes are a-nucleated cells that belong to the series of red blood cells. During the recognition process many data were obtained related to the five types of leukocytes detected with the application. It is important to emphasize that this work does not attempt to diferentiate between smears of leukocytes coming from healthy and sick people; this is because most diseases produce a change in the differential count of leukocytes rather than in theirs forms.

In this way we could get the segmented cells. Now the nuclei and its corresponding membrane are segmented. Color Atlas of Clinical Hematology. Calle 51 A y ave 5 de septiembre. In order to differentiate white blood cells in the microscope, it is needed to process the samples and apply a specific stain. On the other hand, neutrophils and eosinophils have a greater size than basophils, and have a clear differentiation in the amount of cytoplasm with respect to the monocytes and lymphocytes.

The result is shown in Fig.